Identifying Salmonella Choleraesuis Essay Example for Free

Identifying Salmonella Choleraesuis Essay The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop slide, and morphology identification. The unknown bacterium, which was contaminated with Serratia marcescens, was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria, had the appearance of circular form, convex elevation, entire margin, elongated cocci. The tests than showed that the bacteria was gram-negative, non-motile, and was identified as a Salmonella species bacteria, Salmonella choleraesuis. Introduction When applying biology to real world cases, it takes numerous tests to be able to identify exactly what a type of bacteria an unidentified bacterium is, whether it is a bacterium that is causing a person to be ill or a bacterium that is being studied in a laboratory. Often times a bacteria is taken from a contaminated sample that may contain more than one type of bacteria and therefore the specific bacteria that is to be identified has to be isolated. This can often be done by streaking the sample to single colonies. The individual colonies can then be put through several different tests that allow one to identify distinctive aspects of the bacteria. Some examples of these tests are a gram-stain and an API test. These tests allow one to figure out what properties a bacteria strain contains, such as gram negative or gram positive with the gram staining test, or what bacteria an unknown is based on positives and negative of the API test. The results of API tests, will show extremely accurately what a bacteria is based on previous tests performed. One such bacteria than can be identified is Salmonella choleraesuis. There have been many studies done where the particular strain of Salmonella needed to be identified so that a vaccine could be found. In one such study, the strain of Salmonella Choleraesuis had to be identified in order to then use live Salmonella choleraesuis to reduce the seroprevalence and the number of Salmonella carrier pigs at slaughter (Schwarz et al, 2011). By being able to identify the specific strain of the species Salmonella, scientists were about to prevent Salmonella from being passed on through pigs because of the Salmonella choleraesuis vaccine. Identification of a specific bacteria can help scientists make so many improvements in getting rid of deadly bacteria. Materials and Methods This lab was performed over several different days in order to utilize several different techniques in identifying the unknown bacteria. A tube that contained a red species S. marcescens and a white unknown bacteria was streaked to single colonies onto an agar plate using an inoculating loop. The plate was placed in the 37Â ° incubator for 24 hours. Once incubation had occurred the single colonies were used for the different microbiology lab techniques. The first of these techniques used was to determine what the morphology of the unknown bacteria was. The second of these tests was a gram stain. This test was done in order to determine if the unknown bacteria was gram negative or gram positive. The third technique used was a hanging drop slide, which was prepared using another single colony and a light microscope at 100x resolution, in order to observe the bacteria’s motility. The fourth test used was an oxidase test, which was also performed using another single colony of the unknown bacteria. This was done by placing the bacterial colony on filter paper and adding drops of oxidase reagent. The filter paper was then observed to see if it changed blue or not, in order to see if the bacteria produced cytochrome c oxidase. The final test used in the experiment was an API test. To begin the API test, a solution with bacteria and 5 mL of sterile saline, had to be made with a turbidity the same as the McFarland No. 3 (BaSO4) standard. This was done by adding loopfuls of bacteria to the saline solution, mixing the solution on the vortex, and then comparing the turbidity to the McFarland No. 3 standard, until the tubes were both at the same cloudiness. This created solution was then used in the API test by adding specified amounts to each of the microtubes on the API strip. For each of the microtubes whose names were not underlined or boxed, the tubes were filled to where the microtubes met the capsule. In the microtubes whose names were underlined, the microtubes were slightly underfilled, and then the capsule was filled with mineral oil in order to create and anaerobic environment. The last of the microbes were the ones whose names were boxed. In each of these the microtube and the capsule were filled all the way up with the bacteria. The API test strip was then placed in the 37Â °C incubator for 20 hours. After this time, observations were made about each of the different microtubes based on a given summary of results chart for the API test. A select number of microtubes had to have a few reagents added such as 10% ferric chloride, Barritt’s A and B solutions, Kovacs’ reagent, nitrate test reagent, zinc, and hydrogen peroxide, in order to see how the specific microtubes reacted at that time. Once the API test was performed, the negatives and positives found for each of the microtubes was used to identify the unknown bacteria using an identification table that showed the positives and negatives of many known bacterias. Results In the first part technique used in the lab, the morphology of the unknown bacteria colonies was found to be circular form, convex elevation, and entire margin. The second technique used, gram-staining, produced pink bacteria, which had the appearance of elongated cocci. The pink color indicated that the unknown bacteria was gram-negative. In the third technique, the hanging drop slip, there was not true motility and instead only Brownian movement. In the fourth technique, the oxidase test, the filter paper did not turn blue. In the final test, the API test, the results (Fig. 1) shows that the unknown bacteria being studied was Salmonella species, or more specifically, Salmonella choleraesuis. (Fig. 1) The API strip results after 20 hours of incubation at 37Â °C Discussion Through the use of the API test, the results most accurately showed that the bacteria was Salmonella species. This is what the bacteria was, however, it was a specific kind of Salmonella, S. choleraesuis. In the results of the tests, there were four different microtubes that contained different results than in the identification table. In each of the these cases, the API test showed positive results when the table showed negative results. Just because these didn’t match exactly, didn’t mean that the experiment didn’t work right. Each organism is different and because of this will have different reactions at times. In each of the cases, there was a small percentage that the results would be different than the identification table said it would be. With that, the Salmonella species all together was able to be identified better because, in general the different strains of Salmonella each come from the same species. There could also have been slight error when the mi crotubes were filled up. There may have been times when the tubes were overfilled or underfilled or air bubbles may have gotten into the tubes changing the results. Overall, the results were fairly accurate since the Salmonella species was able to be identified. The other tests used in the experiment were also useful in helping identify that S. choleraesuis was the unknown bacteria. It can be shown through previous tests that S. choleraesuis and Salmonella strains have similar characteristics to those found from the techniques used in this lab. In several cases it is shown that Salmonella strains are gram-negative (Kempf et al, 2012). The fact that S. choleraesuis fit the characteristics of previous studies helps show that bacteria was found correctly when preforming the different techniques. References Agbor, T. A., McCormick, B. A. (2011). Salmonella effectors: Imortant players modulating host cell function during infection. Cell Microbiology , 13 (12), 1858-69. Schwarz, P., Kich, J. D., Kolb, J., Cardoso, M. (2011). Use of an avirulentlive Salmonella Choleraesuis vaccine to reduce the prevalence of Salmonella carrier pigs at slaughter. Vet Rec , 21 (169), 553.

The Darknet And The Future Information Technology Essay

The Darknet And The Future Information Technology Essay People have always copied things. In the past, most items of value were physical objects. Patent law and economies of scale meant that small scale copying of physical objects was usually uneconomic, and large-scale copying (if it infringed) was stoppable using policemen and courts. Today, things of value are increasingly less tangible: often they are just bits and bytes or can be accurately represented as bits and bytes. The widespread deployment of packet-switched networks and the huge advances in computers and codec-technologies has made it feasible (and indeed attractive) to deliver such digital works over the Internet. This presents great opportunities and great challenges. The opportunity is low-cost delivery of personalized, desirable high-quality content. The challenge is that such content can be distributed illegally. Copyright law governs the legality of copying and distribution of such valuable data, but copyright protection is increasingly strained in a world of programmab le computers and high-speed networks. The dramatic rise in the efficiency of the darknet can be traced back to the general technological improvements in these infrastructure areas. At the same time, most attempts to fight the darknet can be viewed as efforts to deprive it of one or more of the infrastructure items. Legal action has traditionally targeted search engines and, to a lesser extent, the distribution network. As we will describe later in the paper, this has been partially successful. The drive for legislation on mandatory watermarking aims to deprive the darknet of rendering devices. We will argue that watermarking approaches are technically flawed and unlikely to have any material impact on the darknet. Finally, most content protection systems are meant to prevent or delay the injection of new objects into the darknet. Based on our first assumption, no such system constitutes an impenetrable barrier, and we will discuss the merits of some popular systems. We see no technical impediments to the darknet becoming increasingly efficient (measured by aggregate library size and available bandwidth). However, the darknet, in all its transport-layer embodiments, is under legal attack. In this paper, we speculate on the technical and legal future of the darknet, concentrating particularly, but not exclusively, on peer-to-peer networks. The rest of this paper is structured as follows. Section 2 analyzes different manifestations of the darknet with respect to their robustness to attacks on the infrastructure requirements described above and speculates on the future development of the darknet. Section 3 describes content protection mechanisms, their probable effect on the darknet, and the impact of the darknet upon them. In sections 4 and 5, we speculate on the scenarios in which the darknet will be effective, and how businesses may need to behave to compete effectively with it. 2 The Evolution of the Darknet We classify the different manifestations of the darknet that have come into existence in recent years with respect to the five infrastructure requirements described and analyze weaknesses and points of attack. As a system, the darknet is subject to a variety of attacks. Legal action continues to be the most powerful challenge to the darknet. However, the darknet is also subject to a variety of other common threats (e.g. viruses, spamming) that, in the past, have lead to minor disruptions of the darknet, but could be considerably more damaging. In this section we consider the potential impact of legal developments on the darknet. Most of our analysis focuses on system robustness, rather than on detailed legal questions. We regard legal questions only with respect to their possible effect: the failure of certain nodes or links (vertices and edges of the graph defined above). In this sense, we are investigating a well known problem in distributed systems. 2.1 Early Small-Worlds Networks Prior to the mid 1990s, copying was organized around groups of friends and acquaintances. The copied objects were music on cassette tapes and computer programs. The rendering devices were widely-available tape players and the computers of the time see Fig. 1. Content injection was trivial, since most objects were either not copy protected or, if they were equipped with copy protection mechanisms, the mechanisms were easily defeated. The distribution network was a sneaker net of floppy disks and tapes (storage), which were handed in person between members of a group or were sent by postal mail. The bandwidth of this network albeit small by todays standards was sufficient for the objects of the time. The main limitation of the sneaker net with its mechanical transport layer was latency. It could take days or weeks to obtain a copy of an object. Another serious limitation of these networks was the lack of a sophisticated search engine. There were limited attempts to prosecute individuals who were trying to sell copyrighted objects they had obtained from the darknet (commercial piracy). However, the darknet as a whole was never under significant legal threat. Reasons may have included its limited commercial impact and the protection from legal surveillance afforded by sharing amongst friends. The sizes of object libraries available on such networks are strongly influenced by the interconnections between the networks. For example, schoolchildren may copy content from their family network to their school network and thereby increase the size of the darknet object library available to each. Such networks have been studied extensively and are classified as interconnected small-worlds networks. [24] There are several popular examples of the characteristics of such systems. For example, most people have a social group of a few score of people. Each of these people has a group of friends that partly overlap with their friends friends, and also introduces more people. It is estimated that, on average, each person is connected to every other person in the world by a chain of about six people from which arises the term six degrees of separation. These findings are remarkably broadly applicable (e.g. [20],[3]). The chains are on average so short because certain super-peers have many links. In our example, some people are gregarious and have lots of friends from different social or geographical circles.. We suspect that these findings have implications for sharing on darknets, and we will return to this point when we discuss the darknets of the future later in this paper. The small-worlds darknet continues to exist. However, a number of technological advances have given rise to new forms of the darknet that have superseded the small-worlds for some object types (e.g. audio). 2.2 Central Internet Servers By 1998, a new form of the darknet began to emerge from technological advances in several areas. The internet had become mainstream, and as such its protocols and infrastructure could now be relied upon by anyone seeking to connect users with a centralized service or with each other. The continuing fall in the price of storage together with advances in compression technology had also crossed the threshold at which storing large numbers of audio files was no longer an obstacle to mainstream users. Additionally, the power of computers had crossed the point at which they could be used as rendering devices for multimedia content. Finally, CD ripping became a trivial method for content injection. The first embodiments of this new darknet were central internet servers with large collections of MP3 audio files. A fundamental change that came with these servers was the use of a new distribution network: The internet displaced the sneaker net at least for audio content. This solved several problems of the old darknet. First, latency was reduced drastically. Secondly, and more importantly, discovery of objects became much easier because of simple and powerful search mechanisms most importantly the general-purpose world-wide-web search engine. The local view of the small world was replaced by a global view of the entire collection accessible by all users. The main characteristic of this form of the darknet was centralized storage and search a simple architecture that mirrored mainstream internet servers. Centralized or quasi-centralized distribution and service networks make sense for legal online commerce. Bandwidth and infrastructure costs tend to be low, and having customers visit a commerce site means the merchant can display adverts, collect profiles, and bill efficiently. Additionally, management, auditing, and accountability are much easier in a centralized model. However, centralized schemes work poorly for illegal object distribution because large, central servers are large single points of failure: If the distributor is breaking the law, it is relatively easy to force him to stop. Early MP3 Web and FTP sites were commonly hosted by universities, corporations, and ISPs. Copyright-holders or their representatives sent cease and desist letters to these web-site operators and web-owners citing copyright infringement and in a few cases followed up with legal action [15]. The threats of legal action were successful attacks on those centralized networks, and MP3 web and FTP sites disappeared from the mainstream shortly after they appeared. 2.3 Peer-to-Peer Networks The realization that centralized networks are not robust to attack (be it legal or technical) has spurred much of the innovation in peer-to-peer networking and file sharing technologies. In this section, we examine architectures that have evolved. Early systems were flawed because critical components remained centralized (Napster) or because of inefficiencies and lack of scalability of the protocol (gnutella) [17]. It should be noted that the problem of object location in a massively distributed, rapidly changing, heterogeneous system was new at the time peer-to-peer systems emerged. Efficient and highly scalable protocols have been proposed since then [9],[23]. 2.3.1. Napster Napster was the service that ignited peer-to-peer file sharing in 1999 [14]. There should be little doubt that a major portion of the massive (for the time) traffic on Napster was of copyrighted objects being transferred in a peer-to-peer model in violation of copyright law. Napster succeeded where central servers had failed by relying on the distributed storage of objects not under the control of Napster. This moved the injection, storage, network distribution, and consumption of objects to users. However, Napster retained a centralized database  [1]  with a searchable index on the file name. The centralized database itself became a legal target [15]. Napster was first enjoined to deny certain queries (e.g. Metallica) and then to police its network for all copyrighted content. As the size of the darknet indexed by Napster shrank, so did the number of users. This illustrates a general characteristic of darknets: there is positive feedback between the size of the object library and aggregate bandwidth and the appeal of the network for its users. 2.3.2. Gnutella The next technology that sparked public interest in peer-to-peer file sharing was Gnutella. In addition to distributed object storage, Gnutella uses a fully distributed database described more fully in [13]. Gnutella does not rely upon any centralized server or service a peer just needs the IP address of one or a few participating peers to (in principle) reach any host on the Gnutella darknet. Second, Gnutella is not really run by anyone: it is an open protocol and anyone can write a Gnutella client application. Finally, Gnutella and its descendants go beyond sharing audio and have substantial non-infringing uses. This changes its legal standing markedly and puts it in a similar category to email. That is, email has substantial non-infringing use, and so email itself is not under legal threat even though it may be used to transfer copyrighted material unlawfully. 2.4 Robustness of Fully Distributed Darknets Fully distributed peer-to-peer systems do not present the single points of failure that led to the demise of central MP3 servers and Napster. It is natural to ask how robust these systems are and what form potential attacks could take. We observe the following weaknesses in Gnutella-like systems: Free riding Lack of anonymity 2.4.1 Free Riding Peer-to-peer systems are often thought of as fully decentralized networks with copies of objects uniformly distributed among the hosts. While this is possible in principle, in practice, it is not the case. Recent measurements of libraries shared by gnutella peers indicate that the majority of content is provided by a tiny fraction of the hosts [1]. In effect, although gnutella appears to be a peer-to-peer network of cooperating hosts, in actual fact it has evolved to effectively be another largely centralized system see Fig. 2. Free riding (i.e. downloading objects without sharing them) by many gnutella users appears to be main cause of this development. Widespread free riding removes much of the power of network dynamics and may reduce a peer-to-peer network into a simple unidirectional distribution system from a small number of sources to a large number of destinations. Of course, if this is the case, then the vulnerabilities that we observed in centralized systems (e.g. FTP-serve rs) are present again. Free riding and the emergence of super-peers have several causes: Peer-to-peer file sharing assumes that a significant fraction of users adhere to the somewhat post-capitalist idea of sacrificing their own resources for the common good of the network. Most free-riders do not seem to adopt this idea. For example, with 56 kbps modems still being the network connection for most users, allowing uploads constitutes a tangible bandwidth sacrifice. One approach is to make collaboration mandatory. For example, Freenet [6] clients are required to contribute some disk space. However, enforcing such requirements without a central infrastructure is difficult. Existing infrastructure is another reason for the existence of super-peers. There are vast differences in the resources available to different types of hosts. For example, a T3 connection provides the combined bandwidth of about one thousand 56 kbps telephone connections. 2.4.2 Lack of Anonymity Users of gnutella who share objects they have stored are not anonymous. Current peer-to-peer networks permit the server endpoints to be determined, and if a peer-client can determine the IP address and affiliation of a peer, then so can a lawyer or government agency. This means that users who share copyrighted objects face some threat of legal action. This appears to be yet another explanation for free riding. There are some possible technological workarounds to the absence of endpoint anonymity. We could imagine anonymizing routers, overseas routers, object fragmentation, or some other means to complicate the effort required by law-enforcement to determine the original source of the copyrighted bits. For example, Freenet tries to hide the identity of the hosts storing any given object by means of a variety of heuristics, including routing the object through intermediate hosts and providing mechanisms for easy migration of objects to other hosts. Similarly, Mnemosyne [10] tries to organize object storage, such that individual hosts may not know what objects are stored on them. It is conjectured in [10] that this may amount to common-carrier status for the host. A detailed analysis of the legal or technical robustness of these systems is beyond the scope of this paper. 2.4.3 Attacks In light of these weaknesses, attacks on gnutella-style darknets focus on their object storage and search infrastructures. Because of the prevalence of super-peers, the gnutella darknet depends on a relatively small set of powerful hosts, and these hosts are promising targets for attackers. Darknet hosts owned by corporations are typically easily removed. Often, these hosts are set up by individual employees without the knowledge of corporate management. Generally corporations respect intellectual property laws. This together with their reluctance to become targets of lawsuits, and their centralized network of hierarchical management makes it relatively easy to remove darknet hosts in the corporate domain. While the structures at universities are typically less hierarchical and strict than those of corporations, ultimately, similar rules apply. If the .com and .edu T1 and T3 lines were pulled from under a darknet, the usefulness of the network would suffer drastically. This would leave DSL, ISDN, and cable-modem users as the high-bandwidth servers of objects. We believe limiting hosts to this class would present a far less effective piracy network today from the perspective of acquisition because of the relative rarity of high-bandwidth consumer connections, and hence users would abandon this darknet. However, consumer broadband is becoming more popular, so in the long run it is probable that there will be adequate consumer bandwidth to support an effective consumer darknet. The obvious next legal escalation is to bring direct or indirect (through the affiliation) challenges against users who share large libraries of copyrighted material. This is already happening and the legal threats or actions appear to be successful [7]. This requires the collaboration of ISPs in identifying their customers, which appears to be forthcoming due to requirements that the carrier must take to avoid liability  [2]  and, in some cases, because of corporate ties between ISPs and content providers. Once again, free riding makes this attack strategy far more tractable. It is hard to predict further legal escalation, but we note that the DMCA (digital millennium copyright act) is a far-reaching (although not fully tested) example of a law that is potentially quite powerful. We believe it probable that there will be a few more rounds of technical innovations to sidestep existing laws, followed by new laws, or new interpretations of old laws, in the next few years. 2.4.4 Conclusions All attacks we have identified exploit the lack of endpoint anonymity and are aided by the effects of free riding. We have seen effective legal measures on all peer-to-peer technologies that are used to provide effectively global access to copyrighted material. Centralized web servers were effectively closed down. Napster was effectively closed down. Gnutella and Kazaa are under threat because of free rider weaknesses and lack of endpoint anonymity. Lack of endpoint anonymity is a direct result of the globally accessible global object database, and it is the existence of the global database that most distinguishes the newer darknets from the earlier small worlds. At this point, it is hard to judge whether the darknet will be able to retain this global database in the long term, but it seems seems clear that legal setbacks to global-index peer-to-peer will continue to be severe. However, should Gnutella-style systems become unviable as darknets, systems, such as Freenet or Mnemosyne might take their place. Peer-to-peer networking and file sharing does seem to be entering into the mainstream both for illegal and legal uses. If we couple this with the rapid build-out of consumer broadband, the dropping price of storage, and the fact that personal computers are effectively establishing themselves as centers of home-entertainment, we suspect that peer-to-peer functionality will remain popular and become more widespread. 2.5 Small Worlds Networks Revisited In this section we try to predict the evolution of the darknet should global peer-to-peer networks be effectively stopped by legal means. The globally accessible global database is the only infrastructure component of the darknet that can be disabled in this way. The other enabling technologies of the darknet (injection, distribution networks, rendering devices, storage) will not only remain available, but rapidly increase in power, based on general technological advances and the possible incorporation of cryptography. We stress that the networks described in this section (in most cases) provide poorer services than global network, and would only arise in the absence of a global database. In the absence of a global database, small-worlds networks could again become the prevalent form of the darknet. However, these small-worlds will be more powerful than they were in the past. With the widespread availability of cheap CD and DVD readers and writers as well as large hard disks, the bandwidth of the sneaker net has increased dramatically, the cost of object storage has become negligible and object injection tools have become ubiquitous. Furthermore, the internet is available as a distribution mechanism that is adequate for audio for most users, and is becoming increasingly adequate for video and computer programs. In light of strong cryptography, it is hard to imagine how sharing could be observed and prosecuted as long as users do not share with strangers. In concrete terms, students in dorms will establish darknets to share content in their social group. These darknets may be based on simple file sharing, DVD-copying, or may use special application programs or servers: for example, a chat or instant-messenger client enhanced to share content with members of your buddy-list. Each student will be a member of other darknets: for example, their family, various special interest groups, friends from high-school, and colleagues in part-time jobs (Fig. 3). If there are a few active super-peers users that locate and share objects with zeal then we can anticipate that content will rapidly diffuse between darknets, and relatively small darknets arranged around social groups will approach the aggregate libraries that are provided by the global darknets of today. Since the legal exposure of such sharing is quite limited, we believe that sharing amongst socially oriented groups will increase unabated. Small-worlds networks suffer somewhat from the lack of a global database; each user can only see the objects stored by his small world neighbors. This raises a number of interesting questions about the network structure and object flow: What graph structure will the network have? For example, will it be connected? What will be the average distance between two nodes? Given a graph structure, how will objects propagate through the graph? In particular, what fraction of objects will be available at a given node? How long does it take for objects to propagate (diffuse) through the network? Questions of this type have been studied in different contexts in a variety of fields (mathematics, computer science, economics, and physics). A number of empirical studies seek to establish structural properties of different types of small world networks, such as social networks [20] and the world-wide web [3]. These works conclude that the diameter of the examined networks is small, and observe further structural properties, such as a power law of the degree distribution [5], A number of authors seek to model these networks by means of random graphs, in order to perform more detailed mathematical analysis on the models [2],[8],[21],[22] and, in particular, study the possibility of efficient search under different random graph distributions [18],[19]. We will present a quantitative study of the structure and dynamics of small-worlds networks in an upcoming paper, but to summarize, small-worlds darknets can be extremely efficient for popular titles: very few peers are needed to satis fy requests for top-20 books, songs, movies or computer programs. If darknets are interconnected, we expect the effective introduction rate to be large. Finally, if darknet clients are enhanced to actively seek out new popular content, as opposed to the user-demand based schemes of today, small-worlds darknets will be very efficient. 3 Introducing Content into the Darknet Our analysis and intuition have led us to believe that efficient darknets in global or small-worlds form will remain a fact of life. In this section we examine rights-management technologies that are being deployed to limit the introduction rate or decrease the rate of diffusion of content into the darknet. 3.1 Conditional Access Systems A conditional-access system is a simple form of rights-management system in which subscribers are given access to objects based (typically) on a service contract. Digital rights management systems often perform the same function, but typically impose restrictions on the use of objects after unlocking. Conditional access systems such as cable, satellite TV, and satellite radio offer little or no protection against objects being introduced into the darknet from subscribing hosts. A conditional-access system customer has no access to channels or titles to which they are not entitled, and has essentially free use of channels that he has subscribed or paid for. This means that an investment of ~$100 (at time of writing) on an analog video-capture card is sufficient to obtain and share TV programs and movies. Some CA systems provide post-unlock protections but they are generally cheap and easy to circumvent. Thus, conditional access systems provide a widely deployed, high-bandwidth source of video material for the darknet. In practice, the large size and low cost of CA-provided video content will limit the exploitation of the darknet for distributing video in the near-term. The same can not be said of the use of the darknet to distribute conditional-access system broadcast keys. At some level, each head-end (satellite or cable TV head-end) uses an encryption key that must be made available to each customer (it is a broadcast), and in the case of a satellite system this could be millions of homes. CA-system providers take measures to limit the usefulness of exploited session keys (for example, they are changed every few seconds), but if darknet latencies are low, or if encrypted broadcast data is cached, then the darknet could threaten CA-system revenues. We observe that the exposure of the conditional access provider to losses due to piracy is proportional to the number of customers that share a session key. In this regard, cable-operators are in a safer position than satellite operators because a cable operator can narrowcast more cheaply. 3.2 DRM Systems A classical-DRM system is one in which a client obtains content in protected (typically encrypted) form, with a license that specifies the uses to which the content may be put. Examples of licensing terms that are being explored by the industry are play on these three hosts, play once, use computer program for one hour, etc. The license and the wrapped content are presented to the DRM system whose responsibility is to ensure that: The client cannot remove the encryption from the file and send it to a peer, The client cannot clone its DRM system to make it run on another host, The client obeys the rules set out in the DRM license, and, The client cannot separate the rules from the payload. Advanced DRM systems may go further. Some such technologies have been commercially very successful the content scrambling system used in DVDs, and (broadly interpreted) the protection schemes used by conditional access system providers fall into this category, as do newer DRM systems that use the internet as a distribution channel and computers as rendering devices. These technologies are appealing because they promote the establishment of new businesses, and can reduce distribution costs. If costs and licensing terms are appealing to producers and consumers, then the vendor thrives. If the licensing terms are unappealing or inconvenient, the costs are too high, or competing systems exist, then the business will fail. The DivX DVD rental model failed on most or all of these metrics, but CSS-protected DVDs succeeded beyond the wildest expectations of the industry. On personal computers, current DRM systems are software-only systems using a variety of tricks to make them hard to subvert. DRM enabled consumer electronics devices are also beginning to emerge. In the absence of the darknet, the goal of such systems is to have comparable security to competing distribution systems notably the CD and DVD so that programmable computers can play an increasing role in home entertainment. We will speculate whether these strategies will be successful in the Sect. 5. DRM systems strive to be BOBE (break-once, break everywhere)-resistant. That is, suppliers anticipate (and the assumptions of the darknet predict) that individual instances (clients) of all security-systems, whether based on hardware or software, will be subverted. If a client of a system is subverted, then all content protected by that DRM client can be unprotected. If the break can be applied to any other DRM client of that class so that all of those users can break their systems, then the DRM-scheme is BOBE-weak. If, on the other hand, knowledge gained breaking one client cannot be applied elsewhere, then the DRM system is BOBE-strong. Most commercial DRM-systems have BOBE-exploits, and we note that the darknet applies to DRM-hacks as well. The CSS system is an exemplary BOBE-weak system. The knowledge and code that comprised the De-CSS exploit spread uncontrolled around the world on web-sites, newsgroups, and even T-shirts, in spite of the fact that, in principle, the Digital Millennium Copyright Act makes it a crime to develop these exploits. A final characteristic of existing DRM-systems is renewability. Vendors recognize the possibility of exploits, and build systems that can be field-updated. It is hard to quantify the effectiveness of DRM-systems for restricting the introduction of content into the darknet from experience with existing systems. Existing DRM-systems typically provide protection for months to years; however, the content available to such systems has to date been of minimal interest, and the content that is protected is also available in unprotected form. The one system that was protecting valuable content (DVD video) was broken very soon after compression technology and increased storage capacities and bandwidth enabled the darknet to carry video content. 3.3 Software The DRM-systems described above can be used to provide protection for software, in addition other objects (e.g. audio and video). Alternatively, copy protection systems for computer programs may embed the copy protection code in the software itself. The most important copy-protection primitive for computer programs is for the software to be bound to a host in such a way that the program will not work on an unlicensed machine. Binding requires a machine ID: this can be a unique number on a machine (e.g. a network card MAC address), or can be provided by an external dongle. For such schemes to be strong, two things must be true. First, the machine ID must not be virtualizable. For instance, if it is trivial to modify a NIC driver to return an invalid MAC address, then the software-host binding is easily broken. Second, the code that performs the binding checks must not be easy to patch. A variety of technologies that revolve around software tamper-re

Case Study: Patient with Heart Burn

Case Study: Patient with Heart Burn The patient is a 45 year old male with a history of eight weeks complaint of heart burn that worsens with lying down after dinner. The patient states that the pain lasts thirty minutes to two hours, and that it wakes him at night. Patient reports taking Maalox and Rolaids up to five times daily, however these only help for a short time. Medical History No Known Allergies Hypertension for two years Rheumatoid arthritis in hands and feet for one year Social History Patient is married with three children. Patient drinks two beers daily for the past twenty years and smokes one pack per day for twenty years. Current medications Prednisone 20 mg orally daily Diltiazem 240 mg orally daily Piroxicam 30 mg orally daily Multi- vitamin one orally daily Physical examination T. = 99 RR=18 Pulse =77 BP = 127/76 Weight 242 Height 59† ENT EOMI, PERRLA, mild sputum Cardiac – normal S1 and S2 Chest slight dyspnea Abdomen – soft, with mild pain (4 on 1-10 scale, with 10 being worst) to upper quadrant below sternum. Genitourinary deferred Rectal- deferred Extremities arthritic changes to hands Skin normal Neuro AAO times 3, normal DTR Gastroesophageal reflux disease is a condition in which stomach content leaks backward from the stomach into the esophagus. The symptoms are heartburn, nausea, and regurgitation; this causes an inflammation of the esophagus and possibly the larynx. Complications can be esophageal ulcers, Barrett esophagitis, chronic cough, and can lead to infection and inflammation of the lungs. Diagnosis is typically made by symptoms, endoscopy, and esophageal acid tests (Brunton, Chabner, Knollman, 2011). GERD is caused by a break down in the barrier between the esophagus and the stomach. There are three main types of treatment that physicians consider when treating reflux disease. The treatment will depend on the severity of the symptoms (Brunton, Chabner, Knollman, 2011). With any patient suffering from GERD, there are lifestyle changes and dietary changes that are effective in management of the disease (Brunton, Chabner, Knollman, 2011). Lifestyle changes that the patient can do are as follows: Stop smoking if tobacco products are used (Brunton, Chabner, Knollman, 2011) Taking antacids (of aluminum hydroxide content) (PubMed, 2012) Avoid food consumption within 2 hours of going to bed (PubMed, 2012) Avoid caffeinated beverages, chocolate, nicotine, alcohol, and peppermint (PubMed). Monitor intake of spicy and acidic foods (PubMed, 2012) Limit the amount of high fatty foods in the diet (Brunton, Chabner, Knollman, 2011) Eat 5 to 6 small meals during the day, instead of 3 large meals (PubMed, 2012) Monitor weight, as obesity increases the symptoms of GERD (Brunton, Chabner, Knollman, 2011) Elevate the head of the bed using a 6 to 8 inch block or a wedge under the mattress to enhance acid clearance when reclining (PubMed, 2012) Eat slowly and chew food thoroughly to enhance digestion (PubMed, 2012) *Avoid restrictive clothing, lifting heavy objects, straining, working in a bent position, and stooping (PubMed, 2012). * Chewing gum, this increases saliva production and natural acid reduction (PubMed, 2012). The symptoms may be controlled with the medications; however, the medical issue never goes away. Lifestyle changes are helpful in the overall treatment and control (Brunton, Chabner, Knollman, 2011). Diagnosis Diagnosis and treatment consist of a physical examination and history, esophageal motility testing, esophageal acid test, endoscopy (esophago-gastro-duodenoscopy or EGD), and possible biopsy to diagnosis Barrett’s (Ananal, 2012). The esophageal acid test is considered the â€Å"gold standard† for diagnosis (PubMed). Current medications and interactions related to GERD Calcium channel blockers are classifications of medications used to treat hypertension. These drugs can weaken the lower esophageal sphincter, which can cause GERD (Hughes, Lockart, Joyce, 2007) Corticosteroids in increased amounts have been associated with indigestion, heartburn, stomach pain and cramping, vomiting and diarrhea (Ananal, 2012). NSAIDs such as Piroxicam may cause abdominal cramping or severe pain, severe continuous heart burn and nausea. In combination with corticosteroids there is a higher risk of abdominal pain and the risk of stomach bleeding (Brunton, Chabner, Knollman, 2011). Treatment Treatment can include elevation of the upper body when sleeping, diet changes, and avoid certain foods, eating smaller more frequent meals, and stop smoking and alcohol use (Brunton, Chabner, Knollman, 2011). Medical treatment will include antacids taken one hour after meals; Tums and Rolaids are not recommended for regular use due to being a calcium carbonate. Histamine antagonist, such as Zantac, should be taken 30 minutes before bedtime to prevent nocturnal acid breakthrough. Proton pump inhibitors, such as Prilosec, should be taken, one hour before the morning meal. Foam barriers such as Gaviscon, work as a barrier to stomach acids and may be taken as needed for symptom relief, three times daily (Ananal, 2012). Antacids and alginic acid (Gaviscon) are the drugs of choice for quick relief of symptoms. These agents act primarily by rapidly increasing the ph of the gastric refluxate. Alginic acid reacts with sodium bicarbonate in saliva to form sodium alginates. The sodium alginate floats on the top of the gastric contents where it acts as a mechanical barrier, minimizing exposure of the esophagus to refluxate. H2 receptor blockers act by inhibiting histamine stimulation of the gastric parietal cells, thereby suppressing gastric acid secretion. They are most effective in suppressing nocturnal acid secretion. Proton pump inhibitors (PPI) strongly inhibit gastric acid secretion. They act by irreversibly inhibiting the H+ K+ adenosine Triphosphatase pump of the parietal cell. By blocking the final common pathway of gastric acid suppression compared with H2 receptor blockers. When prescribed appropriately to patients with severe symptoms or refractory disease, the PPIs are more cost effective bec ause of their high healing and remission rate and the consequent prevention of complications (Brunton, Chabner, Knollman, 2011). Prescriptions #1 DATE__6/12/2014_____ Patient name________ (M/F) AGE__45____ Address____________ Weight__242____ Prilosec 40 mg tablet # 30 Take one tablet by mouth every morning before the morning meal for acid reflux control Refill__1__ signature______________ Generic sub __Yes__ DEA#__________ #2 DATE__6/12/2014_____ Patient name________ (M/F) AGE__45____ Address____________ Weight__242____ Zantac 75 mg tablet # 30 Take one tablet by mouth every night 1 hour before bedtime for acid reflux control Refill__1__ signature______________ Generic sub __Yes__ DEA#__________ #3 DATE__6/12/2014_____ Patient name________ (M/F) AGE__45____ Address____________ Weight__242____ Gaviscon 1 chewable tablet as needed for heartburn relief # 1 bottle Take one tablet by mouth three times daily as needed for relief of breakthrough heartburn Refill__1__ signature______________ Generic sub __Yes__ DEA#__________ References Ananal, B. (2012). Peptic ulcer disease. Retrieved from Medscape.com: http://www.medscape.com/article/181753 Brunton, L., Chabner, B., Knollman, B. (2011). Goodman Gilmans:The pharmacological basis of therapeutics (12 ed.). McGraw-Hill. Hughes, J., Lockart, J., Joyce, A. (2007). Do calcium antagonists contribute to gastro-oesophageal reflux disease and concomitant noncardiac chest pain? The British Pharmacological Society Journal. doi:10.1111/j.1365-2125.2007.02851.x PubMed. (2012). Lifestyle changes as a treatment of gastroesophgeal reflux disease:A survey of general practitioners. Retrieved from Pubmed.com: http://www.ncbi.nlm.nih.gov/pmc/article/PMC1661628 PubMed. (n.d.). Gastroesophageal reflux disease. Retrieved from PubMed health: http://www.nebi.nlm.nih.gov/pubmedhealth/PMH6001311 Identification of Didanosine | Results Identification of Didanosine | Results DISCUSSION The procured sample of didanosine was tested for its identification. The drug sample showed compliance with the data given in B.P. and Clarkes which reflects its quality and purity. Quality and purity of sample was also confirmed by the manufacturer. The lipids such as soyalecithin and cholesterol and all other excipients provided by the supplier confirmed by their identification test official in USP 24, IP and EP. All the excipients showed results in compliance with standard specifications. STANDARD CALIBRATION CURVE OF DIDANOSINE From the scanning of drug in 7.4 pH phosphate buffer was concluded that the drug had ÃŽ »max of 249 nm. From the standard calibration curve of drug, it was concluded that drug obeys Beer-Lamberts law in concentration range of 0-20mcg/mL. R2 = 0.9995 Correlation coefficient values indicated the linear correlation between concentration and absorbance. PREPARATION AND CHARACTERIZATION OF LIPOSOMES Among the various methods thin film hydration method is widely used on a laboratory scale. In this method the lipids are casted as stacks of film from their organic solution using flash rotary evaporator under reduced pressure and then the film is dispersed in an aqueous medium. This method yields the liposomes with a heterogeneous size distribution. Also the liposomes that are formed are multilamellar in nature with some unilamellar vesicles. (Vyas and Khar, 2002). Venkataram et al., 1990 have used PC to prepare liposomes in a drug to lipid ratio of 1: 10. The temperature during preparation has been kept 40à ¯Ã¢â‚¬Å¡Ã‚ °C as the glass transition temperature of PC is very low. The drug entrapment into the liposomes depends mainly on Drug: Lipid ratio. In this study, the cholesterol and PC are selected as lipids in combination on basis of percent drug entrapped and rigidity (stability). S.Vemuri et al are stated that, cholesterol improves the fluidity of the bilayer membrane, reduces the permeability of water soluble molecules throught the membrane, and improve the stability of bilayer membrane in the presence of biological fluids such as blood/plasma. The hydration characteristic of lipid film was studied for different batches of liposomes and then liposomes were evaluated for % drug entrapment. From results of % drug entrapped of different batches of liposomes that were prepared and stability of liposomes under different temperature condition it was observed that as the percentage of cholesterol was increased there was subsequent increase in the stability and rigidity of liposomes but at the same time percentage drug entrapment reduced, Due to reduction in phosphatydilcholine. Formulation F4 containing 70% of PC and 30% cholesterol showed maximum drug entrapment (29.41%) with satisfactory stability and rigidity i.e. showed spherical shape with only 1.86% of drug leaching at 25 after 30 days. However, when PC was further increased to 80% keeping cholesterol to 20% there was increase in % drug entrapment but simultaneous decrease in stability and rigidity. The shape was lost and drug leaching was more i.e.10.25%. The data was also treated statistically by using one way analysis (ANOVA) and found to be satisfactory significant difference (P PREPARATION AND CHARACTERIZATION OF DIDANOSINE PROLIPOSOMES The proliposomes of Didanosine were prepared by powder thin film hydration (New, 1990). Here modified rotary evaporator unit was used as described by Lee et al., 1999. Different carriers like lactose, sodium chloride and lactose beads were used for preparation of proliposomes. Were hydrated with distilled water and the liposomes was analysed for % drug entrapped for different batches of proliposomes derived liposomes were as shown in the table 5.3. The Lipid: Carrier ratio was kept 1: 10 as reported by Song et al., 2002. The proliposomes of lactose were quite free flowing compared to the lactose that was used to prepare them and lactose proliposomes showed highest % drug entrapment (29.17). They were less sticky. Also as the amount of lipid i.e. lecithin was increased the proliposomes powder was found to be very sticky. This is because the lipid is sticky at room temperature. In case of sodium chloride the carrier was very free flowing but the proliposomes powder was very sticky compared to that made with either lactose or sorbitol. The particle size of the liposomes formed in sodium chloride and lactose beads was also greater than that formed with lactose. Here the carrier is non porous so majority of the lipid has to be deposited over the on the surface of the carrier, thereby maximizing the possibility of agglomeration and also because sodium chloride is hygroscopic. So it can be said that such nonporous carriers are suitable only for high melting lipids. The results are in accordance with those observed by Payne et al. As the amount of lipid was increased in case of sodium chloride the proliposomes were found to be extremely sticky because they tend to agglomerate (Payne et al., 1986a). The entrapment of the proliposomes made by using sodium chloride as a carrier was very low compared to that of either lactose or lactose beads because of the effect of the monovalent cation Na+. Sodium ion has the effect of increasing the release of cyclosporine from the liposome hence it decreases the entrapment of drug in the liposomes (Al-Angary et al., 1995). The proliposomes of lactose beads were found to be very free flowing just like the lactose beads from which the proliposomes were prepared but the surface area available for coating less compared to lactose powder and sodium chloride so film formed is thick so it yields multilammelar liposomes. Based on the above results the carrier was finalized. In subsequent experiments Lactose was used as a carrier and the formulation was optimized by a 32 factorial design. The effect of the two independent variables viz. Drug: Lipid ratio and Lipid: Carrier ratio was studied on dependant variables like entrapment and mean particle size. All other processing factors like vacuum applied, speed of rotation of round bottom flask; temperature, amount of surfactant etc were kept constant. OPTIMIZATION OF PROLIPOSOMES A prior knowledge and understanding of the process and the process variables under investigation are necessary for achieving a more realistic model. Based on the results of the preliminary experiments, drug: lipid ratio was found to be a major variable in determining PDE and Lipid: carrier ratio in determining the PMD. Hence, these variables were selected to find the optimized condition for higher PDE and PMD using 32 factorial design. By using 32 factorial designs, 9 batches of didanosine liposomes were prepared varying the two independent variables at three levels as recorded in the table. The prepared batches were evaluated for % drug entrapment (PDE) and mean particle size, which were taken as dependent variables and the results were recorded in the table A substantial high drug entrapment was achieved in liposomes of variable X1 (drug: Lipid = 1:15) and X2 (Lipid: Carrier = 1:15). EVALUATION STUDIES OF DIDANOSINE PROLIPOSOMES A) Microscopy of proliposome The microscopy of proliposomes revealed that the surface was smooth due to the coating of the lipid and some of the particles were agglomerated. The pictures of proliposomes are as shown in Figure. After hydration with deionized water a series of time-lapse photographs of proliposome hydration are as shown below in Figure. Here the formation of liposomes form proliposomes is shown. The results indicate that the process of dissolution/disintegration may occur by a progressive hydration of the lipid surface of the proliposome, taking the form of liposomes ‘budding off’ from the central core of the proliposome until both hydration of the lipid and dissolution of the carrier is complete. Although only an imitation of the process of proliposome hydration (due to absence of hand shaking to aid proliposome dispersion), this approach was thought to offer a reasonable indication of the process (Payne et al., 1986b). Finally the liposomes are formed that are multilamellar with a heterogeneous size distribution. The photographs reveal the multilamellar nature (Figure). Also the Maltice crosses shown in Figure indicate that the vesicles are multilamellar in nature. Also there are many unilamellar vesicles. B) Scanning electron micrographs. The scanning electron micrographs of carriers and optimized batch of proliposomes made with different carriers were taken and are as shown in the Figure. From the SEM pictures it is evident that after coating the surface becomes somewhat smooth and the surface defects are no more visible and a thick coating is also seen in proliposomes compared to the carrier alone. After coating of lipid on the surface the particles looks quite opaque and smooth compared to the carrier. EVALUATION STUDIES OF PROLIPOSOME DERIVED LIPOSOMES A) % Drug entrapped By using 32 factorial designs, 9 batches of didanosine liposomes were prepared varying the two independent variables. Various methods have been reported for determination of drug content in liposomes that involve separation of free drug from liposomes either by centrifugation or by dialysis or by sephadex column. Here the separation was affected by sephadex G25 column as reported by Guo et al., 2001. Here the PDE was calculated from the difference between the initial drug added and the drug detected after separation of the free drug. The results of various batches are as shown in the table. It was found that the % drug entrapped was highest when the Drug: Lipid ratio was highest. Also the Lipid: Carrier ratio was found to affect the entrapment of the drug into the liposomes. It was found that the highest % entrapment for all three levels of X2 was obtained at +1 level of X1 that is 21.37% at –1 level, 26.73% at 0 level and 30.89% at +1 level of X2. Graphical presentation figure shows the effect of the independent variable (drug: lipid ratio) on % drug entrapped. The results indicate that as the drug to lipid ratio increases the entrapment of the drug in the liposomes increases, as didanosine is a hydrophilic drug, which finds place within the core. B) Particle size analysis Particle size analysis results of various batches of proliposome derived liposomes are as shown in the table. The results are expressed as particle mean diameter. The particle size of the liposomes decreases as the amount of the carrier increases because there is a greater surface area available for thin lipid film formation which gives rise to a small particle size compared to a thick film that is formed when the amount of carrier is decreased (Hwang et al., 1997). The graph (figure) represents the relationship between lipid: carrier ratio on mean particle size. Mean particle size decreases as the lipid: carrier ratio increases because as the surface area increases thin film formation occurs that gives rise to smaller particle size. C) Stability Studies The optimized formulation was subjected to stability studies at 40C, 250C, 450C for 60 days. They were evaluated for physical appearance, entrapment efficiency, drug content etc. All the results obtained are within the limits and no major changes were identified physically.

Womens Basketball Association :: Creating Writing Fantasy Essays

Women's Basketball Association Background/Prologue [the future]: It is the year 2010. The WNBA has dropped the 'N' from its name and is now referred to as the WBA. There is some confusion over whether the 'W' refers to Women's or World, since both are used. A tiny line of script beneath the official logo on the WBA website gives the name as the Women's Basketball Association, but World Basketball Association is as apt a name as the other. Unlike the NBA, a strictly national league, the WBA made the decision in the early '00s to begin aggressively expanding. The WBA now has a team in most major American and international cities. The league's growth isn't really surprising given the crop of players over the past decade or so. A few have even risen to superstar status, regularly wowing packed arenas with a combination of style, showmanship and skill that hasn't been witnessed in basketball since the glory days of the men's league. The Plot [The past—'90s]: What really kick-started the stellar growth of the WBA was the discovery of Theresa "The Tiger" Tyson. Theresa iss a high school dropout from West Philly who managed to catch the eye of ---- State recruiter Jerry Krieger (on his way back from a game at Penn) during a fierce pickup game. Being as impressed by her obvious sense and no-nonsense attitude as he was with her deadly hook shot, Krieger manages to finesse a deal with his school. If Tyson gets her high school diploma and stays out of trouble (she was a bit of a hell-raiser), they'd manage to find a place for at ----. Theresa is a motivated young woman, if nothing else. Basketball seems to be her only shot at a decent life for herself and her family. As of now, her future does not look too bright, given her lack of education or marketable skills. Not to mention the fact that she is the oldest of five and one of eight people overflowing a two bedroom apartment in the projects. She works the nightshift at a convenience store, protected by a two-inch layer of bulletproof glass and watched by security cameras trained on the register. Her boss does not trust her because she is African-American and poor. She spends the days sleeping, watching TV and keeping an eye on her siblings and seventy seven year old grandmother. Women's Basketball Association :: Creating Writing Fantasy Essays Women's Basketball Association Background/Prologue [the future]: It is the year 2010. The WNBA has dropped the 'N' from its name and is now referred to as the WBA. There is some confusion over whether the 'W' refers to Women's or World, since both are used. A tiny line of script beneath the official logo on the WBA website gives the name as the Women's Basketball Association, but World Basketball Association is as apt a name as the other. Unlike the NBA, a strictly national league, the WBA made the decision in the early '00s to begin aggressively expanding. The WBA now has a team in most major American and international cities. The league's growth isn't really surprising given the crop of players over the past decade or so. A few have even risen to superstar status, regularly wowing packed arenas with a combination of style, showmanship and skill that hasn't been witnessed in basketball since the glory days of the men's league. The Plot [The past—'90s]: What really kick-started the stellar growth of the WBA was the discovery of Theresa "The Tiger" Tyson. Theresa iss a high school dropout from West Philly who managed to catch the eye of ---- State recruiter Jerry Krieger (on his way back from a game at Penn) during a fierce pickup game. Being as impressed by her obvious sense and no-nonsense attitude as he was with her deadly hook shot, Krieger manages to finesse a deal with his school. If Tyson gets her high school diploma and stays out of trouble (she was a bit of a hell-raiser), they'd manage to find a place for at ----. Theresa is a motivated young woman, if nothing else. Basketball seems to be her only shot at a decent life for herself and her family. As of now, her future does not look too bright, given her lack of education or marketable skills. Not to mention the fact that she is the oldest of five and one of eight people overflowing a two bedroom apartment in the projects. She works the nightshift at a convenience store, protected by a two-inch layer of bulletproof glass and watched by security cameras trained on the register. Her boss does not trust her because she is African-American and poor. She spends the days sleeping, watching TV and keeping an eye on her siblings and seventy seven year old grandmother.

Brave New World Ultimate Destruction :: Brave New World

Brave New World Ultimate Destruction    In Brave New World, Aldous Huxley tries to convey the belief that every invention or improvement for the â€Å"betterment† of mankind is only an instrument for his ultimate destruction. â€Å"We are,† he said, â€Å"on the horns of an ethical dilemma and to find the middle way will require all out intelligence and all out good will.† This goes for all fields of life, medical, technical, social, etc. Not only in the book, but also in real life, one can see that this belief is evidently true.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   A first example in the book is the process in which babies are â€Å"born.† The intricate fertilizing, decanting, and conditioning processes is directly used to produce and control a 5 caste system in society. Now, this is not a bad idea, other system is flawed. We see this in people like Bernard. An alpha is supposed to be at the top of society being well formed, tall, good looking and intelligent. Bernard however is somewhat shorter and less handsome than the rest of the men in his caste, and therefore is thought of as queer. This inconsistency in the hatching system shows proof that the system is not completely safe or stable, and will in time produce more and more â€Å"social rejects† that can only lead to destroy the system.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The conditioning process itself is also a good example of how innovation and â€Å"progress† can lead to the destruction of man. John got this in his belief that everyone, no matter how old, was an infant because of the conditioning systems. To truly mature, he thought, one must face suffering and constant cleansing (leading him to his time at the lighthouse). The acceptance and use of the notion that society should be organized by the pleasures of the people is preposterous in that by only living for simple physical pleasure at whatever moment in time it may be, one is stripped of the ability to strive for long-range, true happiness. By breeding a complete society of infants with no concept of planning for a final goal can only end in destruction as it con not support itself if the hand that rocks the cradle, excuse the pun, would happen to waiver.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Thirdly, in the book, the rationing and use of soma as a release for all people is a big red flashing light on the board of pending social disaster.

How Duty of Care Contributes to Safe Practise Essay

What duty of care means in children and young people settings Duty of care – it’s required we have to give the right amount of attention and caution to avoid negligence which would lead to harm to other people. Duty of care is the legal term for safeguarding yourself and others. Children (especially young children) are vulnerable because they have not yet developed the physical and cognitive skills to care for themselves, so they need care from the adults around them. As a practitioner we have a duty of care towards them, the younger and more vulnerable the child, the greater the duty of care needed. Duty of Care includes the following concepts: †¢ to keep individuals safe †¢ to keep individuals free from harm †¢ to give choice As a practitioner, vigilance and attention keeps young children safe as they develop, these areas help when the child has a good role model to teach them: †¢ The ability to foresee and cope with potential dangers †¢ More robust immune systems †¢ Empathy – understand that their actions may hurt or upset others †¢ Communication skills to be able to talk about the harm others may be doing them How this contributes to the safeguarding or protection of individuals Duty of care contributes to the safeguarding/protection of children/young people by keeping them safe and protecting them from abuse, whether this is in a sexual, physical, neglect or emotional harm. Children have a right to be safe and to be treated with respect and dignity, as a practitioner we have to take reasonable steps to ensure the safety and well-being of all children in our care. If we don’t follow all necessary steps it could be regarded as professional neglect, we must always act and be seen to act in the child’s best interests. Babies and under 3’s are in need of an adults care for protection because they are unable to do this for themselves. Duty of care safeguards children in my setting in various ways: * Risk assessments – having done risk assessments we are taking precautions to avoid accidents or the spreading of infections. * Following the correct procedures if we have any concerns for a child’s well-being. * We set clear expectations and boundaries depending on age, stage and development and we discourage any behaviour which could result in a child being harmed or upset. * Assessments and observations on children can alert us to any problems that may need our attention and will also help in the discussions with parents and other professionals. * Always listen to what the children have to say and take any concerns they may have seriously.

To Kill A Mockingbird Chapter Summaries

Chapter 2-3 Dill goes back to his hometown.. ]em and scout have first day of skool ms. Caroline tries to give money to Walter Cunningham for lunch but he never has lunch he's supa poor scout gets in trouble for being able to read and write then burris ewell makes ms. Caroline cry scout wants to drop outta skool but makes a compromise wit atticus that she will go to skool if they keep reading everynight. Chapter 4 Scout thinks something is missing and school wasn't really in her future. She finds 2 pieces of gum near the Radley property but Jem makes her spit it out then the next day they find two pennies and keep them†¦Scout and Calpurnia are getting along. Mrs. Henry Lafayette Dubose meanest old lady ever. Jem roles scout down the road ina tire she falls out and Jem acts tuff and gets the tire out off boo's yard then they play Boo Radley (reinactment) Jem says boo radly is dead but that makes scout nervous because she knows he isnt Sent from my Verizon Wireless BlackBerry Chapt er 5 Dill purposed to Scout then forgot about it a little later in the summer. Dill and Jem excluded Scout. Scout became closer to Ms. Maudie Atkinson. (Widow Scout describes as chameleon lady, hates her house and gardens. ) Scout and Ms.Maudie talk about the Bible. The 3 kids try to dangle a fishing pole with a note on it saying come out to Boo Radley, but Atticus gets mad. Chapter 6 It is the last day Dill is in Maycomb, and at night they decide to try to catch a glimpse of Boo Radley, but they see a shadow and run away. While they are running, Scout trips and makes a gunshot noise (or Boo shoots not sure) they try to meet at a tree near school but Jem's pants get stuck on a fence and have to lie to the adults that he lost them in strip poker to Dill. During the night he goes and gets them even though Scout tells him not too.Chapter 7 Scout is now in 2nd grade and Jem tells her that the night he went back to Radley Place for his pants, they had been sewed messily and folded like s omeone knew he would come back. They also found a ball of grey string in the hiding spot they discovered. The next day they found 2 pieces of soap with images carved in the shape of a boy & a other in a crude dress. They realize the images carved in are themselves. They are trying to figure out who did it. Two weeks later they find a whole pack of gum. Four days later they found a broken pocket watch with an aluminum knife.They write a thank-you note to the giver, but Nathan Radley had cemented in the whole saying it was dying but it was really healthy. Chapter 8 Scout says her and Jem haven't been obeying their dad, Atticus. Mrs. Radley died but no one really cared. It snows for the first time and Scout is scared because she's never seen snow. They have a snow day and no one but them seems to like the snow. They make a large snow man filled with dirt because they don't have much snow. Ms. Maudie's house catches on fire along with Ms. Rachel's but only Ms. Maudie's burns down. Someh ow a mysterious blanket appears around Scout's shoulders.It is implied that Boo Radley put it there. Jem comes clean about all their secrets. They return Ms. Maudie's hat and hedge clipper that they had borrowed to diguise the snowman. Chapter 9 Scout gets mad because Cecil Jacobs says Atticus defends blacks (in court). Scout keeps trying to get out of going to school. Atticus only tells her yes (he does defend a black) and his name is Tom Robinson (a good friend of Cal's). He doesn't want to tell Scout what the case is. He says he took the case to keep respect. Scout talks about Cousin Ike. Uncle Jack stays with them for a week and gives them air rifles.Then, they visit Finch's landing for Christmas. Francis, her annoying cousin, also gives her trouble over Atticus taking Tom Robinson's case. Chapter 10 Atticus is very old (50) unlike the childrens' classmates. He has a â€Å"boring† Job. He never drinks or anything so he isn't very inconspicous. Atticus says its a sin to ki ll a mockingbird because all they do is sing. Scout tries to shoot Ms. Maudie's butt, so Atticus goes over to warn her. The kids go hunting and find Tim a hurt old dog, so they want to help him, but Cal calls around to warn people about the mad dog.The ops come and everyone locks themselves inside. Someone hands Atticus a gun to shoot the dog but he refuses. He ends up shooting the dog and his kids cannot believe he shot the dog. They find out that Atticus' nickname was old one shot. Ms. Maudie tells the kids he gave up hunting because he thought god gave him an unfair advantage. Chapter 1 1 Jem and Scout hate Mrs. Dubose and think she keeps a pistol concealed, and they tried not to past her house. Atticus says Mrs. Dubose is old and sick and not to let her get to Jem. Jem and Scout go to town to buy himself a little steam engine and Scout a aton.But, Jem gets very mad when she says Atticus isn't any better than the n*ggers he defends. Scout pulls him away. When they walk back home, Mrs. Dubose wasnt on the porch. Jem snatched her baton and ran through her yard and left only when he ruined all of her camellia flower bushes, he broke Scouts baton. Atticus gets mad at Jem for ruining Mrs. Dubose's garden and makes him go apologize to her. Atticus says he couldn't go to church if he didn't take Tim Robinson's case. Mrs. Dubose has Jem clean up her yard and says every saturday he has to work on her yard and every ay except Sundays tor a month ne nas to read to her.Scout describes ner as very ugly. While he was reading, she began to become distant (NOT in a good way). They ask if she is okay. Atticus tells Scout what a n*gger lover is. They realize that the alarm clock has been going off a little later each day. They finally finish all the reading and are very happy. Mrs. Dubose dies and Atticus brings a candy box home from her for Jem. Atticus wrote her will and she broke herself from her morphine addiction right before she died. She died free as the â€Å"mounta in air† the box has a white camellia in it. He yells â€Å"Oh hell devil! Atticus wanted Jem to see how courageous she was and courage isn't a man with a gun it is someone that knows they are going to lose but still goes through with whatever they are doing. Chapter 12 Jem is 12 and Scout realizes she shouldn't bother him. Jem tells her to be a girl. Summer comes and Dill doesn't come because he has a new father that he will be spending time with him. He starts reading the paper and all that. Cal says the kids can go to church with her, they are excited. Everyone is respectful of the group, except for Lulu, who asks why Cal brought white kids to a n*gger church.No instruments or books inside, Just a wooden fan for everyone. Even though the kids have dimes, they take Cal's. The priest introduces them and says, â€Å"you all know their father. † They count the money and say no one can leave until they have $10 to help Tim's family. The priest says that Atticus was the ch urch's best friends. Cal tells Scout that Tim is in Jail for supposedly raping Mr. Ewell's girl. Scout doesn't know what a rape is. The kids find out that no one from the church can read and that Cal's supposed birthday is on christmas. Cal worked on Finch's Landing since she could ork and has always worked for the Finchs.Cal told the kids that she spoke like she was black with her neighbors at church so she wouldn't be out of place. Chapter 13 Aunt Alexandra visits. Her and Atticus decided it would be good for Scout to have a feminine role model. Atticus comes back and tells the kids that he thinks it is a good idea for Auntie to live with them, but Scout knows it was Auntie's idea. She became secretary to a Maycomb club. The town is so small and everyone married in it, so Scout says everyone looks alike. Auntie tells Atticus to do and he does tell the kids to live up to the Finch name.Scout and Jem feels like Atticus isn't the same person, but he changes back because Aunt Alexandr a told him to. Chapter 14 When Jem and Scout are going through town, people keep making comments and someone says something about rape, so when Atticus got home Scout asked him what rape was. Atticus says rape is â€Å"carnal knowledge of a female by force and without consent. † She asks him why Cal wouldn't tell her what rape was if that was all it was. Scout asks if she can go to Cal's house, but Auntie sharply says no. Scout over hears Auntie saying they should get rid of Cal. Scout and Jem brawl but Atticus eparates them.Scout thinks there's a snake under her bed but it turns out to be Dill. He hates his new father and tells them that a nearby farmer had been feeding him. Chapter 1 Dill scout and Jem follow atticus to town where they find him sitting in front of Tom robbinson's Jail cell, he waited until a huge mob of people cam in one of the people being the cunningham father. Scout starts sauing things about mr. Cunningham's son, and she gets the whole entire mob to lea ve tom alone without even realizing that she did so Chapter 16 The kids and Atticus go out in the night. In the morning Atticus says BraxtonUnderwood is a negro-hater, and Aunt Alexandra tells him not to say that infront of Cal, but Atticus says she probably knows and she also knows how much she means to them. Scout has noticed a change in Atticus over the past three days. They talk about how even though Mr. Cunningham was ready to kill Atticus with the mob they are still friends and the mob people are still people otherwise Scout couldn't have stopped them. Scout says when Walter comes back to school she's going to kill him, but Atticus tells her NOT to. Atticus tells the kids not to go downtown that day. (NOTE: pg 218 good quote atticus' parenting and profession) To Kill A Mockingbird Chapter Summaries Chapter 2-3 Dill goes back to his hometown.. ]em and scout have first day of skool ms. Caroline tries to give money to Walter Cunningham for lunch but he never has lunch he's supa poor scout gets in trouble for being able to read and write then burris ewell makes ms. Caroline cry scout wants to drop outta skool but makes a compromise wit atticus that she will go to skool if they keep reading everynight. Chapter 4 Scout thinks something is missing and school wasn't really in her future. She finds 2 pieces of gum near the Radley property but Jem makes her spit it out then the next day they find two pennies and keep them†¦Scout and Calpurnia are getting along. Mrs. Henry Lafayette Dubose meanest old lady ever. Jem roles scout down the road ina tire she falls out and Jem acts tuff and gets the tire out off boo's yard then they play Boo Radley (reinactment) Jem says boo radly is dead but that makes scout nervous because she knows he isnt Sent from my Verizon Wireless BlackBerry Chapt er 5 Dill purposed to Scout then forgot about it a little later in the summer. Dill and Jem excluded Scout. Scout became closer to Ms. Maudie Atkinson. (Widow Scout describes as chameleon lady, hates her house and gardens. ) Scout and Ms.Maudie talk about the Bible. The 3 kids try to dangle a fishing pole with a note on it saying come out to Boo Radley, but Atticus gets mad. Chapter 6 It is the last day Dill is in Maycomb, and at night they decide to try to catch a glimpse of Boo Radley, but they see a shadow and run away. While they are running, Scout trips and makes a gunshot noise (or Boo shoots not sure) they try to meet at a tree near school but Jem's pants get stuck on a fence and have to lie to the adults that he lost them in strip poker to Dill. During the night he goes and gets them even though Scout tells him not too.Chapter 7 Scout is now in 2nd grade and Jem tells her that the night he went back to Radley Place for his pants, they had been sewed messily and folded like s omeone knew he would come back. They also found a ball of grey string in the hiding spot they discovered. The next day they found 2 pieces of soap with images carved in the shape of a boy & a other in a crude dress. They realize the images carved in are themselves. They are trying to figure out who did it. Two weeks later they find a whole pack of gum. Four days later they found a broken pocket watch with an aluminum knife.They write a thank-you note to the giver, but Nathan Radley had cemented in the whole saying it was dying but it was really healthy. Chapter 8 Scout says her and Jem haven't been obeying their dad, Atticus. Mrs. Radley died but no one really cared. It snows for the first time and Scout is scared because she's never seen snow. They have a snow day and no one but them seems to like the snow. They make a large snow man filled with dirt because they don't have much snow. Ms. Maudie's house catches on fire along with Ms. Rachel's but only Ms. Maudie's burns down. Someh ow a mysterious blanket appears around Scout's shoulders.It is implied that Boo Radley put it there. Jem comes clean about all their secrets. They return Ms. Maudie's hat and hedge clipper that they had borrowed to diguise the snowman. Chapter 9 Scout gets mad because Cecil Jacobs says Atticus defends blacks (in court). Scout keeps trying to get out of going to school. Atticus only tells her yes (he does defend a black) and his name is Tom Robinson (a good friend of Cal's). He doesn't want to tell Scout what the case is. He says he took the case to keep respect. Scout talks about Cousin Ike. Uncle Jack stays with them for a week and gives them air rifles.Then, they visit Finch's landing for Christmas. Francis, her annoying cousin, also gives her trouble over Atticus taking Tom Robinson's case. Chapter 10 Atticus is very old (50) unlike the childrens' classmates. He has a â€Å"boring† Job. He never drinks or anything so he isn't very inconspicous. Atticus says its a sin to ki ll a mockingbird because all they do is sing. Scout tries to shoot Ms. Maudie's butt, so Atticus goes over to warn her. The kids go hunting and find Tim a hurt old dog, so they want to help him, but Cal calls around to warn people about the mad dog.The ops come and everyone locks themselves inside. Someone hands Atticus a gun to shoot the dog but he refuses. He ends up shooting the dog and his kids cannot believe he shot the dog. They find out that Atticus' nickname was old one shot. Ms. Maudie tells the kids he gave up hunting because he thought god gave him an unfair advantage. Chapter 1 1 Jem and Scout hate Mrs. Dubose and think she keeps a pistol concealed, and they tried not to past her house. Atticus says Mrs. Dubose is old and sick and not to let her get to Jem. Jem and Scout go to town to buy himself a little steam engine and Scout a aton.But, Jem gets very mad when she says Atticus isn't any better than the n*ggers he defends. Scout pulls him away. When they walk back home, Mrs. Dubose wasnt on the porch. Jem snatched her baton and ran through her yard and left only when he ruined all of her camellia flower bushes, he broke Scouts baton. Atticus gets mad at Jem for ruining Mrs. Dubose's garden and makes him go apologize to her. Atticus says he couldn't go to church if he didn't take Tim Robinson's case. Mrs. Dubose has Jem clean up her yard and says every saturday he has to work on her yard and every ay except Sundays tor a month ne nas to read to her.Scout describes ner as very ugly. While he was reading, she began to become distant (NOT in a good way). They ask if she is okay. Atticus tells Scout what a n*gger lover is. They realize that the alarm clock has been going off a little later each day. They finally finish all the reading and are very happy. Mrs. Dubose dies and Atticus brings a candy box home from her for Jem. Atticus wrote her will and she broke herself from her morphine addiction right before she died. She died free as the â€Å"mounta in air† the box has a white camellia in it. He yells â€Å"Oh hell devil! Atticus wanted Jem to see how courageous she was and courage isn't a man with a gun it is someone that knows they are going to lose but still goes through with whatever they are doing. Chapter 12 Jem is 12 and Scout realizes she shouldn't bother him. Jem tells her to be a girl. Summer comes and Dill doesn't come because he has a new father that he will be spending time with him. He starts reading the paper and all that. Cal says the kids can go to church with her, they are excited. Everyone is respectful of the group, except for Lulu, who asks why Cal brought white kids to a n*gger church.No instruments or books inside, Just a wooden fan for everyone. Even though the kids have dimes, they take Cal's. The priest introduces them and says, â€Å"you all know their father. † They count the money and say no one can leave until they have $10 to help Tim's family. The priest says that Atticus was the ch urch's best friends. Cal tells Scout that Tim is in Jail for supposedly raping Mr. Ewell's girl. Scout doesn't know what a rape is. The kids find out that no one from the church can read and that Cal's supposed birthday is on christmas. Cal worked on Finch's Landing since she could ork and has always worked for the Finchs.Cal told the kids that she spoke like she was black with her neighbors at church so she wouldn't be out of place. Chapter 13 Aunt Alexandra visits. Her and Atticus decided it would be good for Scout to have a feminine role model. Atticus comes back and tells the kids that he thinks it is a good idea for Auntie to live with them, but Scout knows it was Auntie's idea. She became secretary to a Maycomb club. The town is so small and everyone married in it, so Scout says everyone looks alike. Auntie tells Atticus to do and he does tell the kids to live up to the Finch name.Scout and Jem feels like Atticus isn't the same person, but he changes back because Aunt Alexandr a told him to. Chapter 14 When Jem and Scout are going through town, people keep making comments and someone says something about rape, so when Atticus got home Scout asked him what rape was. Atticus says rape is â€Å"carnal knowledge of a female by force and without consent. † She asks him why Cal wouldn't tell her what rape was if that was all it was. Scout asks if she can go to Cal's house, but Auntie sharply says no. Scout over hears Auntie saying they should get rid of Cal. Scout and Jem brawl but Atticus eparates them.Scout thinks there's a snake under her bed but it turns out to be Dill. He hates his new father and tells them that a nearby farmer had been feeding him. Chapter 1 Dill scout and Jem follow atticus to town where they find him sitting in front of Tom robbinson's Jail cell, he waited until a huge mob of people cam in one of the people being the cunningham father. Scout starts sauing things about mr. Cunningham's son, and she gets the whole entire mob to lea ve tom alone without even realizing that she did so Chapter 16 The kids and Atticus go out in the night. In the morning Atticus says BraxtonUnderwood is a negro-hater, and Aunt Alexandra tells him not to say that infront of Cal, but Atticus says she probably knows and she also knows how much she means to them. Scout has noticed a change in Atticus over the past three days. They talk about how even though Mr. Cunningham was ready to kill Atticus with the mob they are still friends and the mob people are still people otherwise Scout couldn't have stopped them. Scout says when Walter comes back to school she's going to kill him, but Atticus tells her NOT to. Atticus tells the kids not to go downtown that day. (NOTE: pg 218 good quote atticus' parenting and profession)